Chemical defenses of the Caribbean sponges Agelas wiedenmayeri and Agelas conifera

Previous studies have determined that Caribbean reef sponges of the genus Agelas are chemically defended from fish predation by brominated pyrrole alkaloids, and that the compounds responsible for this defense have been elucidated for one species, Agelas clathrodes. In this study, we expand our understanding of chemical defense in this common sponge genus to include the characterization of defensive metabolites in the tissues of Agelas wiedenmayeri and Agelas conifera. Bioassay-directed isolation of defensive metabolites was undertaken using fish feeding assays carried out in laboratory aquaria and in the field. Agelas wiedenmayeri contained the same two major metabolites as Agelas clathrodes, 4,5-dibromopyrrole-2-carboxylic acid (1), and oroidin (2), in addition to a small amount of bromoageliferin (7). The two major metabolites were present at higher concentrations in samples of Agelas wiedenmayeri than in Agelas clathrodes, and their relative concentrations were reversed, with Agelas wiedenmayeri on average containing more 4,5-dibromopyrrole-2-carboxylic acid (1) (2.0 mg/mL) than oroidin (2) (0.8 mg/mL). Agelas conifera contained a mixture of dimeric bromopyrrole alkaloids dominated by sceptrin (3), with <10% each of dibromosceptrin (5), bromoageliferin (7), dibromoageliferin (8), ageliferin (6), and bromosceptrin (4). Mean concentration of sceptrin (3) in sponge tissue was 5.3 mg/mL; this compound deterred feeding of reef fish in aquarium assays at 1.0 mg/mL, the lowest concentration assayed. Sceptrin (3) concentrations were higher in sponges collected in the southern Bahama Islands than those collected in the middle Bahamas, but reasons for this variation remain unclear. The structure-activity relationship of the pyrrole group was investigated by assaying derivatives of the active metabolites. Feeding deterrent activity of the molecule was enhanced by the addition of bromine to the pyrrole group, but not affected by exchange of the heteroatom from N to O or S. Combining an understanding of the structure-activity relationship of Agelas metabolites with an understanding of the variation in these metabolites across the genus may provide insight into the evolution of defensive chemistry in this highly successful taxa of pan-tropical sponges

Nonlinear Response of Orbit Monitors

The LEP Spectrometer is used to determine beam energy by measuring the bending angle of the beam in a dipole magnet, using six beam position monitors (BPMs), which must have an accuracy close to 10-6 m. The BPMs feature an Al block with an elliptical aperture and four capacitive pickup electrodes; their response depends on the pickup geometry, the aperture shape and the size of the beam. The beam size varies from BPM to BPM, which may give shifts of the measured position. We have investigated the implications of such shifts on the Spectrometer performance. We summarise our current understanding of the BPM behaviour using both a computer model of their response and measurements

Pan-Africanism: a contorted delirium or a pseudonationalist paradigm? Revivalist critique

This essaic-article goes against established conventions that there is anything ethno-cultural (and hence national) about the so-called African tribes. Drawing largely from the culture history of precolonial/prepolitical Africans—that is, the Bantu/Cushitic-Ethiopians (Azanians)—the author has demonstrated vividly that far from being distinct ethno-culture national communities, the so-called tribes of African states are better considered subculture groups, whose regional culture practices erstwhile paid tribute to their nation’s main culture center in Karnak. For example, using the culture symbols and practices of some local groups and linking them to the predynastic and dynastic Pharaonic periods, I argued that there is compelling evidence against qualifying Africa’s tribes as distinct ethno-culture national entities. In genuine culture context, I stressed that the Ritual of Resurrection and its twin culture process of the mummification of deceased indigenous Pharaohs tend to suggest that the object of the Bantu/Cushitic-Ethiopians national culture was life (in its eternal manifestation) and then resurrection later, and that there are recurring (culturally sanctioned) ethical examples among the culture custodians of these subculture groups that generally pay tribute to the overarching culture norm. Furthermore, the fact that the Ritual of Resurrection began in the Delta region and ended at the Sources of the Nile, where the spirit of the deceased indigenous Pharaohs was introduced into the spiritual world of their ancestors, contradicts conventional perceptions that ancient Egypt was a distinct national community isolated from precolonial/prepolitical Africa/Azania

Use of Movable Beam Position Monitors for Beam Size Measurements

The use of beam position monitors (BPMs) as non-intercepting emittance monitors has been proposed in 1983 by Miller et al. The emittance measurement relies on the beam size dependency of the BPM signals. It is shown that the original proposal can be improved by using movable BPMs. Changing the BPM position with a stepping motor allows accurately calibrating the beam size measurement. The absolute scale on the beam size measurement is given by the scale of the stepping motor and can be determined in the laboratory and measured in situ. Uncontrolled changes of the beam position can be monitored through the use of a BPM triplet

Measurements of heavy ion beam losses from collimation

The collimation efficiency for Pb ion beams in the LHC is predicted to be lower than requirements. Nuclear fragmentation and electromagnetic dissociation in the primary collimators create fragments with a wide range of Z/A ratios, which are not intercepted by the secondary collimators but lost where the dispersion has grown sufficiently large. In this article we present measurements and simulations of loss patterns generated by a prototype LHC collimator in the CERN SPS. Measurements were performed at two different energies and angles of the collimator. We also compare with proton loss maps and find a qualitative difference between Pb ions and protons, with the maximum loss rate observed at different places in the ring. This behavior was predicted by simulations and provides a valuable benchmark of our understanding of ion beam losses caused by collimation.Comment: 12 pages, 20 figure

Efficiency for the imperfect LHC collimation system

The LHC collimation system requires a high cleaning efficiency in order to prevent magnet quenches due to regular beam diffusion. The cleaning efficiency is significantly reduced due to imperfections of the collimator jaws and the machine optics. Tracking tools have been set up to predict the cleaning efficiency in presence of multiple imperfections. The deterioration of cleaning efficiency is quantified for different errors, including collimator surface non-flatness, collimator alignment errors, beta beating, orbit errors, non-linear field errors, and chromatic effects

A Method for Simultaneous Optimisation of Orbit and Dispersion in Storage Rings

An algorithm for the simultaneous optimisation of orbit and dispersion in a storage ring is presented. Based on orbit and dispersion measurements the algorithm determines the optimal corrector settings in order to simultaneously minimize the r.m.s orbit, the r.m.s dispersion and the r.m.s strenght of the dipoles correctors. A number of different options for error handling of beam position monitors, weighting, and correction have been introduced to ensure the stability of the algorithm in the environment of a large accelerator. Experimental results are presented for the LEP collider demonstrating the efficiency of the method. The use of this correction algorithm for LEP in 1999 allowed achieving about a factor of two smaller vertical emittances than in previous years